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P5363 Sigma

Protein Purification Techniques: A Practical Approach, 2nd ed.

  •  ISBN-10 0-19-963673-7

  •  ISBN-13 978-0-19-963673-0

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publication info   S. Roe, ed., Oxford University Press, 2001, 280 pp., soft cover
mfr. no.   Oxford University Press

Description

General description

Protein purification techniques have evolved with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. This book focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations.

Table of Contents

List of protocols
Abbreviations
1. Purification strategy, Simon D. Roe
2. Getting started, Simon D. Roe
3. Analysis of purity, Dev Baines
4. Clarification techniques, Ian Reed and Duncan Mackay
5. Cell disintegration and extraction techniques, R. H. Cumming and G. Iceton
6. Concentration of the extract, E. L. V. Harris
7. Separation on the basis of chemistry, Lars Hagel
8. Chromatography on the basis of size, P. Cutler
9. Purification by exploitation of activity, S. Angal, P. D. G. Dean, and Simon D. Roe
10. Scale-up considerations, John B. Noble
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