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P5381 Sigma

Phosphoglucose Isomerase from baker's yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

Synonym: D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Unit Definition

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0

Application

Phosphoglucose Isomerase is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose.

The enzyme has been used to convert fructose to glucose while evaluating fructose content in kudzu roots.

Biochem/physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator. The protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

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