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P5400 Sigma

Pectinesterase from orange peel

lyophilized powder, ≥150 units/mg protein

Synonym: Pectin methylesterase, Pectin pectylhydrolase

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Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

1000 units in glass bottle

Unit Definition

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Physical form

Contains (NH4)2SO4 and sodium chloride

Application

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.

The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

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15

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