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P5412 Sigma

o-Phenylenediamine

tablet, 20 mg substrate per tablet

Synonym: 1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates,
vapor density   3.7 (vs air)
vapor pressure   0.01 mmHg ( 25 °C)
InChI Key   GEYOCULIXLDCMW-UHFFFAOYSA-N
form   tablet
bp   256-258 °C
mp   100-102 °C
fluorescence   λex 337 nm; λem 417 nm (after derivatization)
storage temp.   2-8°C

Description

Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
3
Flash Point(F) 
276.8 °F
Flash Point(C) 
136 °C
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

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