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P5431 Sigma

Pectolyase from Aspergillus japonicus

aseptically filled, lyophilized powder, ≥2 units/mg solid

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Description

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

Prepared from P 3026

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Physical form

Lyophilized powder containing lactose

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

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Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
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Peer-Reviewed Papers
15

References

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P5936 Pectolyase from Aspergillus japonicus, plant cell culture tested, lyophilized powder

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