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P5435 Sigma

Monoclonal Anti-CD14−PE antibody produced in mouse

clone UCHM-1, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD14

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 10 μL using 1 × 106 cells
clone   UCHM-1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 55 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... CD14(929)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human thymocytes followed by peripheral blood T cells.

Antigen Background

CD14 is a phosphoinositol-linked, single-chain, human cell surface glycoprotein antigen which is found on most human peripheral blood monocytes, granulocytes and dendritic reticulum cells, and some tissue macrophages. CD14 may also be present on B cells. Soluble forms of CD14 are present in serum and urine. The antigen functions as an acceptor for LPS-binding protein complexes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding. Cryostat sections, post-fixed in formalin can be stained.
3rd Workshop: code no. 203
4th Workshop: code no. M24

Preparation Note

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

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