|Related Categories||Agarose, Agarose Gel, Molecular Biology, Nucleic Acid Electrophoresis, Plant Biotechnology,|
|grade||for molecular biology|
|contains||0.5 μg/mL ethidium bromide|
|storage temp.||room temp|
Precast gels contain 1.0% agarose in 1X TBE buffer with 0.5 ug/ml ethidium bromide.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.
TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.
MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining.1,2,3,4 If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 500 - 1000 base pairs.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style
DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)
Package of 20 individually foil-wrapped gels.
No detected DNase or RNase activity
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)
Certificate of Analysis
Certificate of Origin
Electrophoresis is a method of separation and purification of macromolecules such as nucleic acids (DNA and RNA) and proteins based on the net charge, size and conformation on a matrix. Nucleic acids...
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography
From our library of Protocols, Sigma-Aldrich presents Chromatin Isolation by RNA Purification (ChIRP) Protocol
Keywords: Cell culture, Cell disruption, Chromatin immunoprecipitation, DNA electrophoresis, DNA purification, Electrophoresis, Gas chromatography, High performance liquid chromatography, Instructions, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sequences, Sequencing, Size-exclusion chromatography, Sonication
1. R.E. Farrell Jr. RNA Methodologies: A Laboratory Guide for Isolation and Characterization San Diego, CA , (1993)
2. Krieg, P., ed. A Laboratory Guide to RNA: Isolation, Analysis and Synthesis New York , (1996)
3. Ausubel, F.M., et al. Short Protocols in Molecular Biology 3rd ed., New York , (1995)
4. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor, NY , (1989)
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|S8140||SSPE Buffer, powder blend|
|P5722||Precast Agarose Gels, 20-well (landscape orientation)|
|P6097||Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)|
|N6019||Neutralizing buffer for Southern blots, powder blend|
|N1531||Denaturation Solution for Neutral Southern Transfer|
|G7654||Gel Loading Solution, Type I|
|B6429||Casein Blocking Buffer 10x, concentrate for blocking hybridization blots|
|S2015||SSPE Buffer 20× Concentrate|
|G2526||Gel Loading Buffer, RNAse, none detected|
|M5755||MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers|
|H7033||PerfectHyb™ Plus Hybridization Buffer, sensitive and fast for Northern and Southern blots|
|S8015||SSC Buffer, powder blend|
|P5972||Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)|
|S6639||SSC Buffer 20× Concentrate, Saline-Sodium Citrate buffer, made with ultrapure water|
|P1415||Prehybridization Solution, for Northern and Southern blots|
|C7594||Casein Blocking Buffer, powder for blocking hybridization blots|
|15356||Blotting-Nylon 66 membranes, type B, positive, roll|
|D4522||Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder|
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