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P5472 Sigma

Precast Agarose Gels

8-well (portrait orientation)

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Properties

Related Categories Agarose, Agarose Gel, Molecular Biology, Nucleic Acid Electrophoresis, Plant Biotechnology,
grade   for molecular biology
form   gel form
contains   0.5 μg/mL ethidium bromide
storage temp.   room temp

Description

Components

Precast gels contain 1.0% agarose in 1X TBE buffer with 0.5 ug/ml ethidium bromide.

Application

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining.1,2,3,4 If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.

Suitable for separation of DNA fragments ranging from 500 - 1000 base pairs.

General description

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 8-well, portrait style

Other Notes

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Packaging

Package of 20 individually foil-wrapped gels.

Quality

No detected DNase or RNase activity

Physical form

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

Price and Availability


Molecular Biology Guide

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Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Protocols

Precast Agarose Gels

Product Description Precautions and Disclaimer Storage/Stability Procedure Results Materials
Keywords: Electrophoresis

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Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for:
Keywords: Buffers, Electrophoresis, Polymerase chain reaction, Separation

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. R.E. Farrell Jr. RNA Methodologies: A Laboratory Guide for Isolation and Characterization San Diego, CA , (1993)

2. Krieg, P., ed. A Laboratory Guide to RNA: Isolation, Analysis and Synthesis New York , (1996)

3. Ausubel, F.M., et al. Short Protocols in Molecular Biology 3rd ed., New York , (1995)

4. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor, NY , (1989)

The genomic landscapes of human breast and colorectal cancers. Wood, L.D., et al. Science 318, 1308-11, (2007)

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