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P5475 Sigma

C-CMV-24 Sequencing Primer

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Properties

grade   for molecular biology
shipped in   dry ice
storage temp.   −20°C

Description

Components

The primer is supplied at an initial concentration of 5 uM in 1xTE buffer. The nucleotide sequence is as follows: 5′-TAT-TAG-GAC-AAG-GCT-GGT-GGG-CAC-3′

Application

The N-CMV-30 sequencing primer is a chemically synthesized, 30 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the C-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 818-847 of positive, coding DNA strand of the pFLAG-CMV vectors.

General description

The C-CMV-24 sequencing primer is a chemically synthesized, 24 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG® fusion proteins at the N-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 1116-1139 of negative, non-coding DNA strand of the pFLAG-CMV-1 vector and nucleotides 1073-1096 of negative, non-coding DNA strand of the pFLAG-CMV-2 vector.

Other Notes

(5′-TAT TAG GAC AAG GCT GGT GGG CAC-3′)

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000

Documents

Certificate of Analysis

Protocols & Articles
Related Products

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P5350 N-CMV-30 Sequencing Primer

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