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P5511 Sigma

Protein Kinase A from bovine heart

≥0.4 units/μg protein, lyophilized powder

Synonym: Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart

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Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Packaging

1, 5 mg in glass bottle

Package size based on protein content

10 mg in poly bottle

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Unit Definition

One unit will transfer 1.0 picomole phosphate from γ-32P-ATP to hydrolyzed and partially dephosphorylated casein per min at pH 6.5 at 30 °C in the presence of cyclic AMP.

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Price and Availability


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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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