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P5542 Sigma

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym: 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, 4.6.x.x P-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents,
Quality Level   PREMIUM
form   lyophilized powder
foreign activity   Phopholipase C (lecithinase) ≤1 units/mg protein
  Sphingomyelinase ≤40 units/mg protein
storage temp.   −20°C
Gene Information   Bacillus cereus E33L ... BCZK3513(3026815)

Description

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Packaging

5, 25 units in glass bottle

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Price and Availability


Amplified Detection

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SZ6116000
Protocols & Articles

Articles

GPI Anchored Glycoproteins

Glycosylphosphatidylinisotol (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting they a...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Cancer, Glycosylations, Transduction

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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