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P5611 Sigma

Protein Kinase Protocols

  •  ISBN-10 0-89603-700-2

  •  ISBN-13 978-0-89603-700-7



Related Categories Biochemistry Books, Books and Software, Labware, Proteomics Books
publication info   A.D. Reith, ed., Humana Press, 2001, 304 pp., hard cover
mfr. no.   Humana


General description

Basic and more sophisticated methods are described for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Emphasis is given to critical technical steps that are often omitted from other published methods. This offers tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.

Table of Contents

Protein Kinase-Mediated Signaling Networks: Regulation and Functional Characterization,
Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies,
Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins, K
Two-Dimensional Phosphoamino Acid Analysis,
Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases,
Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry,
Phosphorylation of Smad Signaling Proteins by Receptor Serine/ Threonine Kinases, .
Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate,
JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins: Analysis by Phosphotyrosine Blotting and EMSA,
Assays for Glycogen Synthase Kinase-3 (GSK-3), .
Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements,
Protein Histidine Kinase,
Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System,
Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins, .
Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay,
Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries,
Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of Receptor Targets (CORT) Strategy, .
Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System,
cDNA Expression Cloning and Charaterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System,
Analysis of SH2 Domain—Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance,
Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins,
Isolation and Characterization of "Orphan-RTK" Ligands Using an Integrated Biosensor Approach,

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