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P5813 Sigma

Monoclonal Anti-p53 antibody produced in mouse

clone BP53-12, ascites fluid

Synonym: Anti-Transformation-related protein 53, Anti-TRP53

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein
clone   BP53-12, monoclonal
antibody form   ascites fluid
isotype   IgG2a
mol wt   antigen mol wt 53 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TP53(7157)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human wild-type p53 protein.

General description

Human cancer is a multistep process in which genetic damage to key regulatory genes, known as oncogenes, and tumor-suppressor genes accumulates. The wildtype p53 gene is classified as a tumor suppressor gene. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 geneare the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. In all of these cases, the mutation is found only in the tumor tissue and not in the normal tissue. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.

Mouse monoclonal clone BP53-12 anti-p53 antibody recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins. The antibody is reactive in immunoblotting of SDSPAGE-separated whole cell lysates from human cell lines known to express high levels of the p53 protein, e.g., the human breast cancer line PMC42, but not against normal fibroblasts. Immunohistochemical staining confined to the malignant lesion has been found across a wide range of human malignancies using frozen, methacarn-, modified-formol- or cold formol-saline-fixed, paraffin-embedded tissue sections. However, not all cancer tissues express p53 protein. The antibody recognizes the p53/T complex (SV-40 encoded large T antigen).

Application

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

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