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P5860 Sigma

Protease from Bacillus sp.

liquid, ≥8 U/g

Synonym: Esperase® 8.0L

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Description

Packaging

250 mL in glass bottle

Legal Information

A product of Novozyme corp.

Esperase is a registered trademark of Novozymes Corp.

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Product P5860 is secreted from Bacillus sp. It is a serine-type protease characterized by excellent perfomance at elevated temperature and pH. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. The enzyme has been used to test UV radiation as an environmental friendly, non-thermal technology for sterilizing enzymes used in the food industry.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.

Price and Availability

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
1
RTECS 
CO9550000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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