|Related Categories||Agarose, Agarose Gel, Molecular Biology, Nucleic Acid Electrophoresis, Plant Biotechnology,|
|grade||for molecular biology|
|contains||.5 μg/mL ethidium bromide|
|storage temp.||room temp|
Precast gels contain 1.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.
TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.
MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining.1,2,3,4 If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 500 – 1000 base pairs.
Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays and are supplied in a box of twenty.
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray (fits common horizontal electrophoresis units)
Well orientation: 24-well, arranged as a 2 x 12 well portrait style
DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)
Package of 20 individually foil-wrapped gels.
No detected DNase or RNase activity
Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)
1. R.E. Farrell Jr. RNA Methodologies: A Laboratory Guide for Isolation and Characterization San Diego, CA , (1993)
2. Krieg, P., ed. A Laboratory Guide to RNA: Isolation, Analysis and Synthesis New York , (1996)
3. Ausubel, F.M., et al. Short Protocols in Molecular Biology 3rd ed., New York , (1995)
4. Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor, NY , (1989)
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|S8140||SSPE Buffer, powder blend|
|P5722||Precast Agarose Gels, 20-well (landscape orientation)|
|P6097||Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)|
|N6019||Neutralizing buffer for Southern blots, powder blend|
|N1531||Denaturation Solution for Neutral Southern Transfer|
|G7654||Gel Loading Solution, Type I|
|B6429||Casein Blocking Buffer 10x, concentrate for blocking hybridization blots|
|G2526||Gel Loading Buffer, RNAse, none detected|
|M5755||MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers|
|S0902||SSC Buffer, powder blend|
|H7033||PerfectHyb™ Plus Hybridization Buffer, sensitive and fast for Northern and Southern blots|
|S8015||SSC Buffer, powder blend|
|P5472||Precast Agarose Gels, 8-well (portrait orientation)|
|P1415||Prehybridization Solution, for Northern and Southern blots|
|C7594||Casein Blocking Buffer, powder for blocking hybridization blots|
|15356||Blotting-Nylon 66 membranes, type B, positive, roll|
|18470||Bromothymol Blue sodium salt, for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6)|
|D3287||Deoxyribonucleic acid, single stranded from human placenta|
|D9156||Deoxyribonucleic acid, single stranded from salmon testes, For hybridization|
|D8661||Deoxyribonucleic acid solution from calf thymus, For hybridization|
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