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P6001 Sigma

p-Phenylenediamine

Synonym: 1,4-Benzenediamine, 1,4-Diaminobenzene, 1,4-Phenylenediamine

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Properties

vapor density   3.7 (vs air)
vapor pressure   1.08 mmHg ( 100 °C)
description   anti-fade reagent
InChI Key   CBCKQZAAMUWICA-UHFFFAOYSA-N
assay   98% (HPLC)
bp   267 °C(lit.)
mp   138-143 °C(lit.)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Anti-fade reagent added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.

Biochem/physiol Actions

Electron Donor. Reduces cytochrome  c.

Caution

Darkens in storage.

Packaging

1 kg in glass bottle

50, 100, 250 g in glass bottle

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Heart, Kidney, Musculo-skeletal system
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
3
RTECS 
SS8050000
Flash Point(F) 
230 °F
Flash Point(C) 
110 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the solubility of p-Phenylediamine, Product P6001?  
This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
For what applications is p-Phenylediamine, Product P6001, used?
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?  
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)."
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)

From our library of Articles, Sigma-Aldrich presents US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)
Keywords: Chromatography, Gas chromatography, Purification

Peer-Reviewed Papers
15

References

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