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P6308 Sigma

Protein Methods, 2nd ed.

  •  ISBN-10 0-471-11837-0

  •  ISBN-13 978-0-471-11837-4

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publication info   D.M. Bollag, Wiley-Liss, 1996, 415 pp., comb bound
mfr. no.   Wiley

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General description

Revised and expanded, this edition remains the key source for tested and proven techniques. Four new chapters are devoted to protein purification by ion exchange, gel filtration, and affinity chromatography, and to protein crystallization.

Table of Contents

Preparation for Protein Isolation.
Protein Extraction and Solubilization.
Protein Concentration Determination.
Concentrating Protein Solutions.
Gel Electrophoresis Under Denaturing Conditions.
Gel Electrophoresis Under Nondenaturing Conditions.
Isoelectric Focusing and Two-Dimensional Gel Electrophoresis.
Immunoblotting.
Ion Exchange Chromatography.
Gel Filtration Chromatography.
Affinity Chromatography.
Hanging Drop Crystallization.
Appendices.
Index

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