|Related Categories||Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,|
|mol wt||mol wt 70,000-150,000|
|suitability||suitable for cell culture|
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Molecular weight based on viscosity.
Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates.
Recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
This product has a higher binding site density, but is more viscous than P7280. It is a poly-D-lysine that can be used with cells that digest poly-L-lysine polymers.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.
Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.
The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.
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Certificate of Analysis
Certificate of Origin
From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
BioFiles 2008, 3.8, 12.
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay
Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation
ncreased expression of 5-HT(1B) receptors by Herpes simplex virus gene transfer in septal neurons: New in vitro and in vivo models to study 5-HT(1B) receptor function. Riegert C., et al. Brain Res. Bull. 76, 439-453, (2008)
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