P6407 Sigma

Poly-D-lysine hydrobromide

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym: PDL

Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
sterility   γ-irradiated
product line   BioReagent
form   lyophilized powder
mol wt   mol wt 70,000-150,000
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

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Analysis Note

Molecular weight based on viscosity.

Application

Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates.

Recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

Other Notes

This product has a higher binding site density, but is more viscous than P7280. It is a poly-D-lysine that can be used with cells that digest poly-L-lysine polymers.

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

General description

Poly-lysine polymers are useful molecules to prepare surfaces for cell attachment. Poly-lysine polymers are available in L and D conformations. Poly-L-lysine polymers can be digested by some cell types, whereas poly-D-lysines are stable. Poly-lysine polymers from 70 to 300 kDa provide sufficient attachment site density for good cell adherence. However, higher molecular weight poly-lysine polymers are more viscous and difficult to work with.

The choice of poly-lysine polymer for a specific cell type and application should account for the susceptibility of the polymer to digestion, the required attachment site density and the difficulty in handling.

Price and Availability


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