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P6407 Sigma

Poly-D-lysine hydrobromide

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym: PDL



Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
sterility   γ-irradiated
product line   BioReagent
form   lyophilized powder
mol wt   mol wt 70,000-150,000
suitability   suitable for cell culture
storage temp.   −20°C


Frequently Asked Questions

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Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.


Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.


Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Price and Availability

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Poly-<SC>D</SC>-lysine hydrobromide

average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Poly-<SC>D</SC>-lysine hydrobromide

mol wt 70,000-150,000

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mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Poly-L-Lysine Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 12.


Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
George Sitterley
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Applications, Cell culture, Enzyme-linked immunosorbent assay, Help

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Events, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers


Set your institution to view full text papers.

Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads. Jacobson, B.S. and Branton, D. Science 195, 302, (1977)


Neuroprotection requires the functions of the RNA-binding protein HuR. Skliris A, Papadaki O, Kafasla P, et al. Cell Death Differ. 22(5), 703-18, (2015)


Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Szalai P, Hagen LK, Sætre F, et al. Exp. Cell Res. 333(1), 21-38, (2015)


Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy. Kren BT, Unger GM, Abedin MJ, et al. Breast Cancer Res. 17, 19, (2015)


AMPA receptor desensitization is the determinant of AMPA receptor mediated excitotoxicity in purified retinal ganglion cells. Park YH, Mueller BH, McGrady NR, et al. Exp. Eye Res. 132, 136-50, (2015)


Efficient transfection of dissociated mouse chromaffin cells using small-volume electroporation. Hoerauf WW, Cazares VA, Subramani A, et al. Cytotechnology 67(3), 573-83, (2015)


Label-free cancer cell detection with impedimetric transducers. de la Rica R, Thompson S, Baldi A, et al. Anal. Chem. 81(24), 10167-71, (2009)


ncreased expression of 5-HT(1B) receptors by Herpes simplex virus gene transfer in septal neurons: New in vitro and in vivo models to study 5-HT(1B) receptor function. Riegert C., et al. Brain Res. Bull. 76, 439-453, (2008)


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P7280 Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
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