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P6516 Sigma

Poly-L-lysine hydrobromide Green Alternative

mol wt 4,000-15,000 by viscosity

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Properties

Related Categories Amino Acids, Biochemicals and Reagents, Chemical Synthesis, Greener Alternative Products, Homopolymers,
form   solid
mol wt   mol wt ≤15,000 by MALLS
  mol wt 4,000-15,000 by viscosity
greener alternative product score   old score: 11
new score: 5
Find out more about DOZN™ Scoring.
greener alternative product characteristics   Atom Economy
Less Hazardous Chemical Syntheses
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.
greener alternative category   Re-engineered
storage temp.   −20°C

Description

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Packaging

1 g in poly bottle

25, 100, 500 mg in poly bottle

Preparation Note

This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Bio Brief: Poly-L-lysine Hydrobromide [VIDEO]

Poly-L-lysine hydrobromide is a non-specific attachment factor useful for promoting cell adhesion to solid substrates, such as coverslips, slides, and microwell plates. Poly-L-lysine is a positively-...
Keywords: Adhesion

Peer-Reviewed Papers
15

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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