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P6649 Sigma

Protein A–Sepharose® 6MB

aqueous ethanol suspension

Synonym: Protein A–Agarose macrobeads

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Molecular Biology, Protein A, G, Protein A, G and L Resins,
form   aqueous ethanol suspension
extent of labeling   ~1 mg per mL
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ~6 mg/mL binding capacity (human IgG)
storage temp.   2-8°C

Description

Packaging

1 mL in glass insert

5, 10 mL in glass bottle

Physical form

Suspension in 20% ethanol

Application

Protein A-Sepharose® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
RIDADR 
UN 1993 / PGIII
WGK Germany 
3
Flash Point(F) 
100.4 °F
Flash Point(C) 
38 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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