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  • P6736 - Principles of Fluorescence Spectroscopy, 2nd ed.

P6736 Sigma

Principles of Fluorescence Spectroscopy, 2nd ed.

  •  ISBN-10 0-306-46093-9

  •  ISBN-13 978-0-306-46093-7



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publication info   J.R. Lakowicz, ed., Kluwer Academic/Plenum, 1999, 725 pp., hard cover
mfr. no.   Kluwer Academic Publishers


General description

This edition maintains emphasis on basics, while updating the examples to include more recent results. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of time resolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. Glossaries are provided for commonly used acronyms and mathematical symbols.

Table of Contents

1. Introduction to Fluorescence.
2. Instrumentation for Fluorescence Spectroscopy.
3. Fluorophores.
4. Time-Domain Lifetime Measurements.
5. Frequency-Domain Lifetime Measurements.
6. Solvent Effects on Emission Spectra.
7. Dynamics of Solvent and Spectral Relaxation.
8. Quenching of Fluorescence.
9. Advanced Topics in Fluorescence Quenching.
10. Fluorescence Anistropy.
11. Time-Dependent Anisotropy Decays.
12. Advanced Anisotropy Concepts.
13. Energy Transfer.
14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers.
15. Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions.
16. Protein Fluorescence.
17. Time-Resolved Protein Fluorescence.
18. Excited State Reactions.
19. Fluorescence Sensing
20. Long Lifetime Metal-Ligand Complexes.
21. DNA Technology.
22. Phase Sensitive and Phase Resolved Emission Spectra.
Appendixes: I. Corrected Emission Spectra.
II. Fluorescent Lifetime Standards.
III. Additional Reading Index

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