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P6855 Sigma

Protein A–Gold

~2.5 A520 units/mL, aqueous glycerol suspension, 17-23 nm (monodisperse), 20 nm nominal (colloidal gold)

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Properties

Related Categories Antibodies, Protein A, G and L Detection Reagents, Secondary Antibodies More...
form   aqueous glycerol suspension
concentration   ~2.5 A520 units/mL
particle size   17-23 nm (monodisperse)
nominal   20 nm (colloidal gold)
conjugate   gold conjugate
storage temp.   2-8°C

Description

Other Notes

Protein A, extracellular (P 6031), adsorbed to colloidal gold.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 20% glycerol, and 15 mM sodium azide.

Application

Protein A-Gold can be used in dot blot. It can also be used to detect immunoglobulins.

General description

Protein A is a 42 kD, highly stable cell surface receptor isolated from Staphylococcus aureus which binds selectively and with high affinity to the Fc portion of IgG from various species.Protein A-gold is an electron dense, non-fading marker and can be used as a probe in electron microscopy (TEM and SEM), light microscopy and blotting. Protein A - 20 nm colloidal gold reacts specifically with IgG of human.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
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Documents

Certificate of Analysis

Protocols & Articles

Articles

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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References

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