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  • P6874 - Monoclonal Anti-p53 antibody produced in mouse

P6874 Sigma

Monoclonal Anti-p53 antibody produced in mouse

clone DO-1, purified from hybridoma cell culture

Synonym: Anti-Transformation-related protein 53, Anti-TRP53



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells
clone   DO-1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~53 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TP53(7157)
biological source   mouse
conjugate   unconjugated



recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

General description

(P53) is a tumor suppressor protein that is expressed in normal tissues. It has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity. Monoclonal anti-(p53) antibody can be used in immunoprecipitation and immunohistochemistry. Mouse anti-(p53) antibody reacts specifically with (p53) if human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Monoclonal anti-(p53) antibody can be used in immunoblotting, flow cytometry and immunocytochemistry. It can also be used in ELISA and CHIP assay.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


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Are stem cells involved in cancer? Cancer stem cells (CSC’s) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis1. Cancer resea...
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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