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P6874 Sigma

Monoclonal Anti-p53 antibody produced in mouse

clone DO-1, purified from hybridoma cell culture

Synonym: Anti-Transformation-related protein 53, Anti-TRP53

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells
clone   DO-1, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~53 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TP53(7157)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

General description

(P53) is a tumor suppressor protein that is expressed in normal tissues. It has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity. Monoclonal anti-(p53) antibody can be used in immunoprecipitation and immunohistochemistry. Mouse anti-(p53) antibody reacts specifically with (p53) if human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal anti-(p53) antibody can be used in immunoblotting, flow cytometry and immunocytochemistry. It can also be used in ELISA and CHIP assay.

Price and Availability


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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Cancer Stem Cells: New Targets for Cancer Therapy

Are stem cells involved in cancer? Cancer stem cells (CSC’s) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis1. Cancer resea...
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

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癌症干细胞

传统观点认为,癌症是环境或内部因素诱导正常细胞内的关键致癌基因和肿瘤抑制基因突变导致的一种疾病。当这些突变导致细胞向更原始、高度增殖状态转化,克隆扩张形成白血病或实体肿瘤时,就会出现癌症的临床表现1。然而,这种模式不能够完全解释许多肿瘤和转移瘤的长发育延迟,产生细胞去分化和细胞永生的起始诱导机制,或肿瘤本身内的细胞功能和表型多样性起源。过去十年中,越来越多的证据表明,恶性肿瘤起源于组织干细胞在突...
Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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