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P6988 Sigma

Protein Structure, Stability and Folding

  •  ISBN-10 0-89603-682-0

  •  ISBN-13 978-0-89603-682-6

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publication info   K.P. Murphy, ed., Humana Press, 2001, 264 pp., hard cover
mfr. no.   Humana

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General description

Volume 164 of the Methods in Molecular Biology series describes the newest experimental and theoretical methods. Among the techniques discussed are the methods for calculating aspects of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments.

Table of Contents

Stabilization of Protein Structure, .
Protein Stabilization by Naturally Occurring Osmolytes, .
The Thermodynamic Linkage Between Protein Structure, Stability, and Function,
Measuring the Conformational Stability of a Protein by Hydrogen Exchange,
Modeling the Native State Ensemble,
Conformational Entropy in Protein Folding: A Guide to Estimating Conformational Entropy via Modeling and Computation, .
Turn Scanning: Experimental and Theoretical Approaches to the Role of Turns, .
Laser Temperature-Jump Methods for Studying Folding Dynamics,
Kinetics of Conformational Fluctuations by EX1 Hydrogen Exchange in Native Proteins,
Molecular Dynamics Simulations of Protein Unfolding/Folding, .

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