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P7059 Sigma

Phosphate Buffered Saline

10× PBS for Western blots and IP

Synonym: PBS, Phosphate buffered saline

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Properties

Related Categories Buffers and Reagents, Buffers, Reagents, & Stains, Cell Biology, Dissociation, General Stem Cell Biology,
grade   for molecular biology
sterility   0.2 μm filtered
form   liquid
pH range   7.2 - 7.6 (1x solution)
storage temp.   room temp

Description

Components

10x PBS contains:
• 30mM sodium phosphate
• 1.5 M sodium chloride
• 10.5 mM potassium phosphate

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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