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P7173 Sigma

Pyruvate Carboxylase from bovine liver

buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

Synonym: Pyruvate:CO2 ligase (ADP-forming)

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Properties

Related Categories 6.4.x.x C-C Bonds, 6.x.x.x Ligases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   buffered aqueous glycerol solution
concentration   ≥0.5 mg/mL
foreign activity   lactic dehydrogenase ≤0.5%
storage temp.   −20°C

Description

Preparation Note

Affinity purified

Unit Definition

One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.

Physical form

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

Application

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.

The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.

Biochem/physiol Actions

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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