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P7186 Sigma

PCR: A Practical Approach

  •  ISBN-10 0-19-963196-4

  •  ISBN-13 978-0-19-963196-4

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Related Categories Books and Software, Labware, Molecular Biology Books, PCR Books
publication info   M.J. McPherson, et al., ed., IRL Press at Oxford University Press, 1991, 253 pp., soft cover
mfr. no.   Oxford University Press

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General description

This book covers gene analysis at the level of restriction fragment polymorphism, point mutation, and sequence analysis. Methods are given for using different source materials: cloned DNA, genomic DNA, RNA, nucleic acid from archive material, and PCR products themselves.

Table of Contents

1. Polymerase Chain Reaction: Basic Principles and Automation, Graham R. Taylor
2. Polymerase Chain Reaction in Genetic Diagnosis, A. Ivinson and G.R. Taylor
3. Extraction of Nucleic Acid from Fresh and Archival Material, D. P. Jackson, J.D. Haden, and P. Quirke
4. Analysis of Genomic Sequence Variation Using Amplification and Mismatch Detection and Direct Sequencing, R. G. Roberts et al.
5. Detection of Deletions and Point Mutations, B.J.F. Rossiter, M. Grompe and C.T. Caskey
6. PCR of TG Microsatellites, M. Litt
7. Genome Amplification Using Primers Directed to Interspersed Repetitive Sequences (IRS - PRC), S.A. Ledbetter and D.L. Nelson
8. PCR Amplification of Microdissected DNA, D.H. Johnson
9. Inverse Polymerase Chain Reaction, J. Silver
10. PCR-directed cDNA Libraries, S.J. Gurr and M.J. McPherson
11. PCR with Highly Redundant Primers, M.J. McPherson, K.M. Jones and S.J. Gurr
12. General Application of PCR to Gene Cloning and Manipulation, T. Clackson, D. Gussow, and P.T. Jones
13. Semi-quantitative PCR for the Analysis of Gene Expression, M.J. Dallman and A.C.G. Porter
14. The Fidelity of DNA Polymerases Used in the PCR, K.A. Eckert and T.A. Kunkel

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