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P7306 Sigma

Polyethylene glycol solution

average mol wt 1,450, Hybri-Max, 50 % (w/v), sterile-filtered, BioReagent, suitable for hybridoma

Synonym: PEG solution

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Essential Chemicals, Hybridoma Platform, Hybridoma Reagents,
grade   Hybri-Max
sterility   sterile-filtered
product line   BioReagent
mol wt   average mol wt 1,450
concentration   50 % (w/v)
impurities   endotoxin, tested
suitability   suitable for hybridoma
storage temp.   2-8°C

Description

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Packaging

Packaged in sealed ampules under nitrogen.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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