• USA Home
  • P7397 - Plasminogen from human plasma

EMAIL THIS PAGE TO A FRIEND
P7397 Sigma

Plasminogen from human plasma

lyophilized powder, ≥2 units/mg protein

Synonym: Profibrinolysin

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Coagulation Proteins and Reagents, Enzymes, Inhibitors, and Substrates, Plasma & Blood Proteins,
form   lyophilized powder
mol wt   mol wt 90 kDa
composition   , ~2%
suitability   not suitable for streptokinase determination by clot formation procedure
foreign activity   plasmin <0.2 unit/unit plasminogen
storage temp.   −20°C
Gene Information   human ... PLG(5340)

Description

Analysis Note

ε-Aminocaproic acid free

Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.

Biochem/physiol Actions

Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).

Reconstitution

Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.

Unit Definition

One unit will produce a ΔA275 of 1.0 from α-casein in 20 min at pH 7.5 at 37 °C, when measuring perchloric acid soluble products in a volume of 5.0 mL. Activity determined after activation to plasmin with urokinase.

Physical form

Lyophilized powder containing NaCl, EDTA, lysine and Tris buffer

Physical properties

Native-intact human plasminogen is a 791 amino acid glyco-protein with as many as 24 disulfide bonds. Plasminogen contains a single N-linked sialylated biantennary glycan. The two O-glycans possess a Gal β-1-3GalNAc core which are α-2-3 sialylated at the terminal Gal. An additional disialylated form has a second sialic acid residue with an α-2-6 linkage to GalNAc. Mono- and disialylated forms occur at a molar ratio of 80:20 in human plasminogen.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

replaced by

Product #

Description

Add to Cart

P7999 Plasminogen from human plasma, ≥2.0 units/mg protein, lumps and powder, suitable for streptokinase determination by clot formation procedure

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?