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P7545 Sigma

Pancreatin from porcine pancreas

8 × USP specifications

Synonym: Pancreatin from hog pancreas

Properties

Related Categories Core Bioreagents, Enzymes, Research Essentials More...
contains   lactose or sucrose as extender (The sucrose may contain up to 3.25% starch.)
storage temp.   −20°C

Description

Biochem/physiol Actions

Pancreatin contains enzymatic components including trypsin, amylase and lipase, ribonuclease, and protease, produced by the exocrine cells of the porcine pancreas. This combination of enzymes allows it to hydrolyze proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40°C, will digest not less than 25 times its weight of casein in 60 minutes at pH 7.5 at 40°C and will release not less than microequivalents of acid per min per mg pancreatin from olive oil at pH 9.0 at 37°C.

Due to the enzymatic components such as trypsin, amylase and lipase, pancreatin hydrolyzes proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40 °C, will digest not less than 25 times its weight of casein in 60 minutes at pH7.5 at 40 °C and will release not less than 2 microequivalents of acid per min per mg pancreatin from olive oil at pH9.0 at 37 °C.

Application

Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. It is used for in vitro digestibility analysis1. Product P7545 has been used to test the sensitivities of cellulolytic bacteria inhibitors2.

The enzyme has been used along with amyloglucosidase for the in vitro digestion of starch in food samples. Pancreatin from Sigma has been used for in vitro pepsin-pancreatin digestion assays while estimating of lower gut digestibility using rumen feed residues. It has also been used along with pepsin to simulate in vitro gastric and ileal digestion of raw materials in pigs.

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