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P7545 Sigma

Pancreatin from porcine pancreas

8 × USP specifications

Synonym: Pancreatin from hog pancreas

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Properties

Related Categories Core Bioreagents, Enzymes, Research Essentials More...
contains   lactose as extender
storage temp.   −20°C

Description

Biochem/physiol Actions

Pancreatin contains enzymatic components including trypsin, amylase and lipase, ribonuclease, and protease, produced by the exocrine cells of the porcine pancreas. This combination of enzymes allows it to hydrolyze proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40°C, will digest not less than 25 times its weight of casein in 60 minutes at pH 7.5 at 40°C and will release not less than microequivalents of acid per min per mg pancreatin from olive oil at pH 9.0 at 37°C.

Due to the enzymatic components such as trypsin, amylase and lipase, pancreatin hydrolyzes proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40 °C, will digest not less than 25 times its weight of casein in 60 minutes at pH7.5 at 40 °C and will release not less than 2 microequivalents of acid per min per mg pancreatin from olive oil at pH9.0 at 37 °C.

Application

Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. It is used for in vitro digestibility analysis1. Product P7545 has been used to test the sensitivities of cellulolytic bacteria inhibitors2.

The enzyme has been used along with amyloglucosidase for the in vitro digestion of starch in food samples. Pancreatin from Sigma has been used for in vitro pepsin-pancreatin digestion assays while estimating of lower gut digestibility using rumen feed residues. It has also been used along with pepsin to simulate in vitro gastric and ileal digestion of raw materials in pigs.

Price and Availability

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United States Pharmacopeia (USP) Reference Standard

powder, beige, amylase >100 U/mg

Pancreatin from porcine pancreas

4 × USP specifications

Pancreatin from porcine pancreas

powder, suitable for cell culture, 4 × USP specifications

≥3 × USP specifications

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Hazard Codes (Europe) 
Xn
Risk Statements (Europe) 
Safety Statements (Europe) 
22-24-26-36/37
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Peer-Reviewed Papers

References

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1. Effect of novel maize-based dietary fibers on postprandial glycemia and insulinemia. Kendall, C.W., et al. J. Am. Coll. Nutr. 27, 711-8, (2008)

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2. Competition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria Junqin Chen and Paul J. Weimer Microbiology 147, 21-30, (2001)

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Assessment of novel oral lipid-based formulations of amphotericin B using an in vitro lipolysis model. Ibrahim F, Gershkovich P, Sivak O, et al. Eur. J. Pharm. Sci. 46(5), 323-8, (2012)

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Characterization of Tyr-Leu-Gly, a novel anxiolytic-like peptide released from bovine αS-casein. Mizushige T, Sawashi Y, Yamada A, et al. FASEB J. 27(7), 2911-7, (2013)

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Preparation of human milk fat substitutes from palm stearin with arachidonic and docosahexaenoic acid: combination of enzymatic and physical methods. Zou XQ, Huang JH, Jin QZ, et al. J. Agric. Food Chem. 60(37), 9415-23, (2012)

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Integrity and stability of oral liposomes containing bile salts studied in simulated and ex vivo gastrointestinal media. Hu S, Niu M, Hu F, et al. Int. J. Pharm. 441(1-2), 693-700, (2013)

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In vitro digestion rate and estimated glycemic index of oat flours from typical and high β-glucan oat lines. Kim HJ and White PJ J. Agric. Food Chem. 60(20), 5237-42, (2012)

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A blinded randomised controlled trial to determine the effect of enteric coating on enzyme treatment for canine exocrine pancreatic efficiency. Mas A, Noble PJ, Cripps PJ, et al. BMC Vet. Res. 8, 127, (2012)

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Cytotoxicity of α-dicarbonyl compounds submitted to in vitro simulated digestion process. Amoroso A, Maga G, and Daglia M Food Chem. 140(4), 654-9, (2013)

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Estimation of ruminal degradation and intestinal digestion of tropical protein resources using the nylon bag technique and the three-step in vitro procedure in dairy cattle on rice straw diets. Promkot, C., M. Wanapat, and P. Rowlinson Asian. Australas. J. Anim. Sci. 20(12), 1849, (2007)

Carbon balances for in vitro digestion and fermentation of potential roughages for pregnant sows. Becker, P. M., et al. Anim. Feed Sci. Technol. 110(1), 159-174, (2003)

Effects of porcine pancreatic enzymes on the pancreas of hamsters. Part 2: carcinogenesis studies. Nozawa F, Yalniz M, Saruc M, et al. JOP 13(5), 482-7, (2012)

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Effects of porcine pancreatic enzymes on the pancreas of hamsters. Part 1: basic studies. Saruc M, Nozawa F, Yalniz M, et al. JOP 13(5), 476-81, (2012)

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In vitro induction of nephrogenesis in mouse metanephric mesenchyme with lithium introduction and ureteric bud recombination. Halt K and Vainio S Methods Mol. Biol. 886, 23-30, (2012)

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[Peculiarities of diabetes mellitus course in chronic pancreatitis]. Vinokurova LV, Drozdov VN, Berezina OI, et al. Eksp. Klin. Gastroenterol. (7), 59-63, (2011)

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[Chronic pancreatitis: microbe-intestinal tissue complex and systemic inflammatory response]. Grinevich VB, Sas EI, Denisov NL, et al. Eksp. Klin. Gastroenterol. (7), 13-7, (2011)

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Randomised clinical trial: a 1-week, double-blind, placebo-controlled study of pancreatin 25 000 Ph. Eur. minimicrospheres (Creon 25000 MMS) for pancreatic exocrine insufficiency after pancreatic surgery, with a 1-year open-label extension. Seiler CM, Izbicki J, Varga-Szabó L, et al. Aliment. Pharmacol. Ther. 37(7), 691-702, (2013)

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In vitro study of triglyceride lipolysis and phase distribution of the reaction products and cholesterol: effects of calcium and bicarbonate. Vinarov Z, Petrova L, Tcholakova S, et al. Food Funct. 3(11), 1206-20, (2012)

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Investigation of the susceptibility of acid-deamidated wheat gluten to in vitro enzymatic hydrolysis using Raman spectra and free amino acid analysis. Liao L, Wang Q, and Zhao MM J. Sci. Food Agric. 92(9), 1865-73, (2012)

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Baselines representing blood glucose clearance improve in vitro prediction of the glycaemic impact of customarily consumed food quantities. Monro JA, Mishra S, and Venn B Br. J. Nutr. 103(2), 295-305, (2010)

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Intestinal permeation enhancement of docetaxel encapsulated into methyl-β-cyclodextrin/poly(isobutylcyanoacrylate) nanoparticles coated with thiolated chitosan. Mazzaferro S, Bouchemal K, Skanji R, et al. J. Control. Release 162(3), 568-74, (2012)

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Digestive stability of hydroxytyrosol, hydroxytyrosyl acetate and alkyl hydroxytyrosyl ethers. Pereira-Caro G, Sarriá B, Madrona A, et al. Int. J. Food Sci. Nutr. 63(6), 703-7, (2012)

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Angiotensin-I converting enzyme inhibitory and antioxidant activities of egg protein hydrolysates produced with gastrointestinal and nongastrointestinal enzymes. You SJ and Wu J J. Food Sci. 76(6), C801-7, (2011)

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Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model. Ritter P BMC Microbiol. 9, 87, (2009)

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Release of EPA and DHA from salmon oil - a comparison of in vitro digestion with human and porcine gastrointestinal enzymes. Aarak KE, Kirkhus B, Holm H, et al. Br. J. Nutr. 110(8), 1402-10, (2013)

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Release of flavonoids from lupin globulin proteins during digestion in a model system. Czubinski J, Dwiecki K, Siger A, et al. J. Agric. Food Chem. 60(7), 1830-6, (2012)

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The effect of thermal and ultrasonic treatment on amino acid composition, radical scavenging and reducing potential of hydrolysates obtained from simulated gastrointestinal digestion of cowpea proteins. Quansah JK, Udenigwe CC, Saalia FK, et al. Plant Foods Hum. Nutr. 68(1), 31-8, (2013)

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Ultrasound-assisted enzymatic hydrolysis for iodinated amino acid extraction from edible seaweed before reversed-phase high performance liquid chromatography-inductively coupled plasma-mass spectrometry. Romarís-Hortas V, Bermejo-Barrera P, and Moreda-Piñeiro A J. Chromatogr. A 1309, 33-40, (2013)

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Simultaneous production of multi-functional peptides by pancreatic hydrolysis of bovine casein in an enzymatic membrane reactor via combinational chromatography. Wu S, Qi W, Li T, et al. Food Chem. 141(3), 2944-51, (2013)

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The function of digestive enzymes on Cu, Zn, and Pb release from soil in in vitro digestion tests. Li Y, Demisie W, and Zhang MK Environ. Sci. Pollut. Res. Int. 20(7), 4993-5002, (2013)

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Stereoselective synthesis of D- and L-carbocyclic nucleosides by enzymatically catalyzed kinetic resolution. Mahler M, Reichardt B, Hartjen P, et al. Chemistry 18(35), 11046-62, (2012)

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Metabolic stability of long-acting luteinizing hormone-releasing hormone antagonists. Yao JF, Zhou N, Lv YJ, et al. Amino Acids 43(4), 1557-66, (2012)

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Comparative efficiency of microbial enzyme preparations versus pancreatin for in vitro alimentary protein digestion. Andriamihaja M, Guillot A, Svendsen A, et al. Amino Acids 44(2), 563-72, (2013)

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Selection of dominant NSLAB from a mature traditional cheese according to their technological properties and in vitro intestinal challenges. Papanikolaou Z, Hatzikamari M, Georgakopoulos P, et al. J. Food Sci. 77(5), M298-306, (2012)

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Mechanism of hydrolysis of native and cooked starches from different botanical sources in the presence of tea extracts. Guzar I, Ragaee S, and Seetharaman K J. Food Sci. 77(11), C1192-6, (2012)

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The identification and characterization of chitotriosidase activity in pancreatin from porcine pancreas. Shen CR, Liu CL, Lee HP, et al. Molecules 18(3), 2978-87, (2013)

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Physical, chemical and biochemical properties of casein hydrolyzed by three proteases: partial characterizations. Luo Y, Pan K, and Zhong Q Food Chem. 155, 146-54, (2014)

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Characterisation of different digestion susceptibility of lupin seed globulins. Czubinski J, Dwiecki K, Siger A, et al. Food Chem. 143, 418-26, (2014)

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Merck 14,7006

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