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P7548 Sigma

Protein Function: A Practical approach

  •  ISBN-10 0-19-963007-0

  •  ISBN-13 978-0-19-963007-3

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publication info   T. Creighton, ed., IRL Press, 1990, 360 pp., soft cover
mfr. no.   Oxford University Press

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General description

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

Table of Contents

1. Identifying proteins for proteome studies: a two-dimensional gel electrophoresis approach
2. Expressing cloned genes in Escherichia coli
3. Folding proteins
4. Ligand binding
5. Ligand blotting
6. Affinity labeling
7. Chemical cross-linking and protein function
8. Analysis of sequence-specific DNA-binding proteins
9. Purification of sequence-specific DNA-binding proteins by affinity chromatography
10. Chemical modification

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