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P7607 Sigma

Monoclonal Anti-Protein Phosphatase 1α antibody produced in mouse

clone PPI-377, ascites fluid

Synonym: Anti-PP1α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   monkey, rat, mouse, human, rabbit, bovine
application(s)   immunocytochemistry: suitable
  microarray: suitable
  western blot: 1:500 using mouse fibroblasts cell extract
clone   PPI-377, monoclonal
antibody form   ascites fluid
isotype   IgG2b
mol wt   antigen mol wt 37.5 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPP1CA(5499)
mouse ... Ppp1ca(19045)
rat ... Ppp1ca(24668)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant rabbit protein phosphatase 1α (PP1α) catalytic subunit

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 catalyzes a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1α is expressed in brain, specifically in cerebellum, prefrontal cortex and synapses
Monoclonal Anti-Protein Phosphatase 1α specifically recognizes an epitope within the catalytic subunit of PP1α isoform (37.5 kDa).

Specificity

The antibody reacts with protein phosphatase 1α and recognizes an epitope within its catalytic subunit.

Application

Anti-Protein Phosphatase 1α is suitable for immunoblotting at a working dilution of 1:500 using mouse fibroblasts cell extract. A dilution of 1:200 has been used for immunoblotting in myocardial protein extracts of Göttinger minipigs. For immunoprecipitation, 1 μg antibody/20 μg of pig protein extracts was suitable. The antibody is also suitable for immunocytochemistry and protein microarray.

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