Pyruvate Kinase from rabbit muscle Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein

Properties

Related Categories	2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes I-Z, Glycolysis, Glycolysis Enzymes, Metabolic Pathways, Metabolomics More...
Quality Level	PREMIUM
type	Type VII
form	buffered aqueous glycerol solution
foreign activity	lactic dehydrogenase and creatine phosphokinase ≤0.01%
	phosphoglucomutase and myokinase ≤0.05%
storage temp.	2-8°C

Description

Analysis Note

Protein determined by biuret.

Unit Definition

One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.01 M phosphate, pH 7.0

Application

Pyruvate kinase is an enzyme involved in glycolysis and is involved in gluconeogenesis. Product P7768 is from rabbit muscle and is provided in a buffered aqueous glycerol solution and has been used in PGM enzyme assays to determine phosphoglycerate mutase activity¹. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency².

Biochem/physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP. Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5