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P7777 Sigma

Poly(ethylene glycol)

Hybri-Max, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma

Synonym: PEG

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Essential Chemicals, Hybridoma Platform, Hybridoma Reagents,
grade   Hybri-Max
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
sterility   autoclaved
product line   BioReagent
form   waxy solid
autoignition temp.   581 °F
mol wt   mol wt 1,300-1,600
impurities   endotoxin, tested
viscosity   25-35 cP (210 °F)
mp   40-50 °C
solubility   water: soluble (5 mL water/vial at 45 °C)
suitability   suitable for hybridoma

Description

Application

Recommended for cell fusion.

Reconstitution

Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TQ3500000
Flash Point(F) 
444.2 °F
Flash Point(C) 
229 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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