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P7777 Sigma

Poly(ethylene glycol)

Hybri-Max, mol wt 1,300-1,600, waxy solid, BioReagent, suitable for hybridoma

Synonym: PEG

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Essential Chemicals, Hybridoma Platform, Hybridoma Reagents,
grade   Hybri-Max
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
sterility   autoclaved
product line   BioReagent
form   waxy solid
autoignition temp.   581 °F
mol wt   mol wt 1,300-1,600
impurities   endotoxin, tested
viscosity   25-35 cP (210 °F)
mp   40-50 °C
solubility   water: soluble (5 mL water/vial at 45 °C)
suitability   suitable for hybridoma

Description

Application

Recommended for cell fusion.

Reconstitution

Melt the PEG at 45 °C or higher in a waterbath. Dilute to 30-50% using sterile, pre-warmed serum-free medium (e.g., for a 50% solution add 5 mL to the 5 g vial of PEG). The pH of the solution should be slightly alkaline (pink, not orange or purple). Adjustment of the pH with sterile NaOH may be required. Store solution below 0 °C. Prolonged storage or repeated freezing and thawing is not recommended.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TQ3500000
Flash Point(F) 
444.2 °F
Flash Point(C) 
229 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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