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P7899 Sigma

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, PD-PG, Plant Biology,
biological source   rabbit
antibody form   fractionated antiserum
clone   polyclonal
form   lyophilized powder
species reactivity   horseradish
application(s)   indirect ELISA: 1:100,000-1:200,000
conjugate   unconjugated
storage temp.   2-8°C

Description

Analysis Note

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

Immunogen

Purified peroxidase from horseradish

Reconstitution

Reconstitute with deionized water.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

General description

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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