|Related Categories||Alphabetical Index, Antibodies, Miscellaneous, PD-PG, Plant Biology,|
|antibody form||fractionated antiserum|
|application(s)||indirect ELISA: 1:100,000-1:200,000|
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Purified peroxidase from horseradish
Reconstitute with deionized water.
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
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