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P7956 Sigma

Protein Kinase C from rat brain

>90% (SDS-PAGE), lyophilized powder

Synonym: PKC



Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
assay   >90% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt 82 kDa by SDS-PAGE
color   white
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prkca(24680), Prkcb(25023), Prkcd(170538), Prkce(29340), Prkcg(24681), Prkch(81749), Prkci(84006), Prkcz(25522), Prkd1(85421)


Other Notes

Mixture of isozymes.


Reconstitute with 100 μl of dH2O.

Unit Definition

One unit will transfer 1 nmol of phosphate per min from ATP to histone H1 at pH 7.4 at 30 °C.

Physical form

Supplied as lyophilized white powder containing Tris buffer salts, EDTA, EGTA, DTT, Tween 20, sodium chloride, leupeptin, and sucrose as stabilizer.

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

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Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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