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P7962 Sigma

Monoclonal Anti-PSTAIR antibody produced in mouse

clone PSTAIR, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
species reactivity   Japanese quail, human, Xenopus, ciliates, onion, carp, amago salmon, monkey, mouse, lily, goldfish, eel, starfish
application(s)   immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:4,000 using a COS-7 cell extract
clone   PSTAIR, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 33-34 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CDC2(983)
mouse ... Cdc2a(12534)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.

General description

In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.

Application

Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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