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P7979 Sigma

Anti-Protein Phosphatase 1α antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-PP1α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   human
application(s)   microarray: suitable
  western blot: 1:2,000 using human A431 epidermal carcinoma cell extract.
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 36 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPP1CA(5499)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 316-328 of the human protein phosphatase 1α (catalytic subunit) on a synthetic MAP carrier.

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 catalyzes a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1α is expressed in brain and muscle.
Anti-Serine/threonine protein phosphatase 1α specifically recognizes PP1α isoform and recognizes an epitope within its catalytic subunit (36 kDa) in human, mouse, rat and bovine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

By immunoblotting, the antibody specifically reacts with protein phosphatase 1α and recognizes an epitope within its catalytic subunit. The sequence is identical in rabbit PP1α.

Application

A minimum dilution of 1:2000 of Anti-PP1α is used for detection by immunoblotting in whole cell extracts of A431, human epidermoid carcinoma cell line. The antibody is also suitable for protein microarray.

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