• USA Home
  • P8038 - Proteinase, bacterial

EMAIL THIS PAGE TO A FRIEND

P8038 Sigma

Proteinase, bacterial

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Synonym: Alkaline Protease, Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilo peptidase A

Purchase

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Application

The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart.1 It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.2

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Protocols & Applications

Non-specific Protease Activity Assay Video

Price and Availability

TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
  • . Superior Band Resolution
  • . Strong & Tear Resistant
  • . Higher Capacity Wells



Everything You Need for Cell Culture

Customers Also Viewed

7-15 units/mg protein (biuret), aqueous propylene glycol solution (containing sodium benzoate and potassium sorbate)

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Type VIII, lyophilized powder, 7-15 units/mg solid

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
1
RTECS 
UK9540000

Protocols & Articles

Protocols

Enzymatic Assay of Protease Using Casein As a Substrate

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Related Content

Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.

Find enzymes / proteins, substrates, activators, and inhibitors. Resources for metabolic pathways, kinases, proteolytic enzymes and inhibitors, carbohydrate analysis as well as new cell signaling, an...
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Exercise training decreases rat heart mitochondria free radical generation but does not prevent Ca2+-induced dysfunction. Starnes JW, Barnes BD, and Olsen ME J. Appl. Physiol. 102(5), 1793-8, (2007)

Loading...


2. In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. Naoumov NV, Alexander GJ, Eddleston AL, et al. J. Clin. Pathol. 41(7), 793-8, (1988)

Loading...


Silencing of cardiac mitochondrial NHE1 prevents mitochondrial permeability transition pore opening María C. Villa-Abrille, et al. Am. J. Physiol. Heart Circ. Physiol. 300, H1237-H1251, (2011)

Loading...


Discovery of a new role of human resistin in hepatocyte low-density lipoprotein receptor suppression mediated in part by proprotein convertase subtilisin/kexin type 9. Melone M, Wilsie L, Palyha O, et al. J. Am. Coll. Cardiol. 59(19), 1697-705, (2012)

Loading...


Influenza virus partially counteracts restriction imposed by tetherin/BST-2. Mangeat B, Cavagliotti L, Lehmann M, et al. J. Biol. Chem. 287(26), 22015-29, (2012)

Loading...


[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia]. Tang H Zhonghua Fang She Xue Za Zhi 16(4), 296-300, (1982)

Loading...


Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis. Uehara R, Takeuchi Y, Tanaka S, et al. Biochemistry 51(26), 5369-78, (2012)

Loading...


A His6-SUMO-eXact tag for producing human prepro-urocortin 2 in Escherichia coli for raising monoclonal antibodies. Liew OW, Ang CX, Peh YP, et al. J. Immunol. Methods 403(1-2), 37-51, (2014)

Loading...


Entrapment of subtilisin in ceramic sol-gel coating for antifouling applications. Regina VR, Søhoel H, Lokanathan AR, et al. ACS Appl. Mater. Interfaces 4(11), 5915-21, (2012)

Loading...


An unusual subtilisin-like serine protease is essential for biogenesis of quinohemoprotein amine dehydrogenase. Nakai T, Ono K, Kuroda S, et al. J. Biol. Chem. 287(9), 6530-8, (2012)

Loading...


Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. Ikemura H, Takagi H, and Inouye M J. Biol. Chem. 262(16), 7859-64, (1987)

Loading...


Extending the cellulosome paradigm: the modular Clostridium thermocellum cellulosomal serpin PinA is a broad-spectrum inhibitor of subtilisin-like proteases. O Cuív P, Gupta R, Goswami HP, et al. Appl. Environ. Microbiol. 79(19), 6173-5, (2013)

Loading...


PmSERPIN3 from black tiger shrimp Penaeus monodon is capable of controlling the proPO system. Wetsaphan N, Rimphanitchayakit V, Tassanakajon A, et al. Dev. Comp. Immunol. 41(2), 110-9, (2013)

Loading...


Crystal structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L. Murayama K, Kato-Murayama M, Hosaka T, et al. J. Mol. Biol. 423(3), 386-96, (2012)

Loading...


Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus. Nedkov P, Oberthür W, and Braunitzer G Biol. Chem. Hoppe-Seyler 366(4), 421-30, (1985)

Loading...


Cross talk between the nuclease and helicase activities of Dna2: role of an essential iron-sulfur cluster domain. Pokharel S and Campbell JL Nucleic Acids Res. 40(16), 7821-30, (2012)

Loading...


NaStEP: a proteinase inhibitor essential to self-incompatibility and a positive regulator of HT-B stability in Nicotiana alata pollen tubes. Jiménez-Durán K, McClure B, García-Campusano F, et al. Plant Physiol. 161(1), 97-107, (2013)

Loading...


Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin. Petrillo T, O'Donohoe CA, Howe N, et al. Biochemistry 51(31), 6164-70, (2012)

Loading...


Examining structure-activity correlations of some high activity enzyme preparations for low water media. Solanki K, Gupta MN, and Halling PJ Bioresour. Technol. 115, 147-51, (2012)

Loading...


Enzymatic activation of cellulose acetate membrane for reducing of protein fouling. Koseoglu-Imer DY, Dizge N, and Koyuncu I Colloids Surf. B Biointerfaces 92, 334-9, (2012)

Loading...


Microneme protein 5 regulates the activity of Toxoplasma subtilisin 1 by mimicking a subtilisin prodomain. Saouros S, Dou Z, Henry M, et al. J. Biol. Chem. 287(43), 36029-40, (2012)

Loading...


Characterization of a novel subtilisin-like protease myroicolsin from deep sea bacterium Myroides profundi D25 and molecular insight into its collagenolytic mechanism. Ran LY, Su HN, Zhou MY, et al. J. Biol. Chem. 289(9), 6041-53, (2014)

Loading...


Thrombolytic effects of Douchi fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo. Yuan J, Yang J, Zhuang Z, et al. BMC Biotechnol. 12, 36, (2012)

Loading...


Interaction of counterions with subtilisin in acetonitrile: insights from molecular dynamics simulations. Lousa D, Cianci M, Helliwell JR, et al. J. Phys. Chem. B 116(20), 5838-48, (2012)

Loading...


Cloning of a fibrinolytic enzyme (subtilisin) gene from Bacillus subtilis in Escherichia coli. Ghasemi Y, Dabbagh F, and Ghasemian A Mol. Biotechnol. 52(1), 1-7, (2012)

Loading...


Effects of the KIF2C neck peptide on microtubules: lateral disintegration of microtubules and β-structure formation. Shimizu Y, Shimizu T, Nara M, et al. FEBS J. 280(7), 1681-92, (2013)

Loading...


Coupling of epithelial Na+ and Cl- channels by direct and indirect activation by serine proteases. Gondzik V, Weber WM, and Awayda MS Am. J. Physiol. Cell Physiol. 303(9), C936-46, (2012)

Loading...


In silico characterization of alkaline proteases from different species of Aspergillus. Morya VK, Yadav S, Kim EK, et al. Appl. Biochem. Biotechnol. 166(1), 243-57, (2012)

Loading...


DEVDase activity is induced in potato leaves during Phytophthora infestans infection. Fernández MB, Daleo GR, and Guevara MG Plant Physiol. Biochem. 61, 197-203, (2012)

Loading...


Purification, characterization and identification of a senescence related serine protease in dark-induced senescent wheat leaves. Wang R, Liu S, Wang J, et al. Phytochemistry 95, 118-26, (2013)

Loading...


Assessment of the respiratory sensitization potential of proteins using an enhanced mouse intranasal test (MINT). Krieger SM, Boverhof DR, Woolhiser MR, et al. Food Chem. Toxicol. 59, 165-76, (2013)

Loading...


One-step purification of a functional, constitutively activated form of visual arrestin. Huang L, Mao X, Abdulaev NG, et al. Protein Expr. Purif. 82(1), 55-60, (2012)

Loading...


The hybrid sensor kinase RscS integrates positive and negative signals to modulate biofilm formation in Vibrio fischeri. Kati Geszvain et al J. Bacteriol. 190, 4437-46, (2008)

Loading...


Crystal structure of greglin, a novel non-classical Kazal inhibitor, in complex with subtilisin. Derache C, Epinette C, Roussel A, et al. FEBS J. 279(24), 4466-78, (2012)

Loading...


Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function. Liu D, Wang Y, Wei L, et al. Gene 514(2), 99-104, (2013)

Loading...


Protease inhibitor from insect silk-activities of derivatives expressed in vitro and in transgenic potato. Kodrík D, Kludkiewicz B, Navrátil O, et al. Appl. Biochem. Biotechnol. 171(1), 209-24, (2013)

Loading...


Characterization, cloning, and heterologous expression of a subtilisin-like serine protease gene VlPr1 from Verticillium lecanii. Yu G, Liu JL, Xie LQ, et al. J. Microbiol. 50(6), 939-46, (2012)

Loading...


Proprotein convertase subtilisin/kexin type 9 as transducer of physiologic influences on cellular cholesterol: a case for resistin. Fazio S and Linton MF J. Am. Coll. Cardiol. 59(19), 1706-8, (2012)

Loading...


Comment on Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis. Viqar B, Gopinath A, Dimitrov JD, et al. J. Agric. Food Chem. 60(16), 4170-2, (2012)

Loading...


Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?