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P8045 Sigma

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

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Properties

Related Categories Antibodies, Protein A, G and L Detection Reagents, Secondary Antibodies More...
recombinant   expressed in unspecified host
form   lyophilized powder
composition   Protein, ~90% biuret
extent of labeling   2-4 mol d-biotin per mol protein
application(s)   direct ELISA: 1:70,000 using human IgG
conjugate   biotin conjugate
storage temp.   2-8°C

Description

Other Notes

Truncated - lacks albumin, Fab and membrane binding sites.

Packaging

Package size based on protein content

Preparation Note

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Specificity

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Application

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.

General description

Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Peer-Reviewed Papers
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References

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