|Related Categories||1-1B, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies in Array,|
|species reactivity||human, bovine, canine|
|application(s)||immunocytochemistry: suitable using cultured cells|
|immunohistochemistry (frozen sections): suitable|
|indirect immunofluorescence: 1:1,000 using cultured MDBK cells|
|western blot: 1:2,000 using cultured MDBK cells|
|antibody form||ascites fluid|
|form||buffered aqueous solution|
|mol wt||antigen mol wt 85 kDa|
|contains||15 mM sodium azide|
|shipped in||dry ice|
|Gene Information||human ... JUP(3728)|
recombinant chicken plakoglobin.
Plakoglobin is a desmosomal protein that regulates developmental signalling in vertebrates. Tyrosine phosphorylation of plakoglobin has been linked to adenocarcinomas 2. Plakoglobin may function as a tumor suppressor in ovarian and breast cancers 3. Monoclonal anti-plakoglobin (catenin γ) antibody specifically binds to plakoglobin (85kDa band and a possibly lower band) by immunoblotting. The antibody reacts with plakoglobin in bovines, humans and dogs, but does not react with β catenin.
Monoclonal anti-plakoglobin (catenin γ) antibody is suitable for use in immunocytochemistry (using MDCK cells), immunoprecipitation, and in immunoblotting 1. The antibody may also be used for immunohistochemistry (frozen sections), microarray, western blot (1:500, using cultured MDBK cells) and indirect immunofluorescence (1:1000, using cultured MDBK cells).
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
2. Tyrosine Phosphorylation of β-Catenin and Plakoglobin Enhanced by Hepatocyte Growth Factor and Epidermal Growth Factor in Human Carcinoma Cells Shibamoto, S., et al. Cell Adhes. Commun. 1(4), 295-305, (1994)
3. The human plakoglobin gene localizes on chromosome 17q21 and is subjected to loss of heterozygosity in breast and ovarian cancers. Aberle, H., et al. Proc. Natl. Acad. Sci. U. S. A. 92(14), 6384-8, (1995)
Taxol reduces cytosolic E-cadherin and β-catenin levels in nasopharyngeal carcinoma cell line TW-039: cross-talk between the microtubule- and actin-based cytoskeletons. Chen, H., et al. J. Cell. Biochem. 79, 542-556, (2000)
Ordered assembly of the adhesive and electrochemical connections within newly formed intercalated disks in primary cultures of adult rat cardiomyocytes. Geisler, S.B., et al. J. Biomed. Biotechnol. 2010, 624719, (2010)
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