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Protocols for Neural Cell Culture, 3rd ed.

  •  ISBN-10 0-89603-902-1

  •  ISBN-13 978-0-89603-902-5

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Related Categories Books and Software, Cell Culture Books, Cell Signaling Books, Labware
publication info   S. Fedoroff and A. Richardson, ed., Humana Press, 2001, 352 pp., soft cover
mfr. no.   Humana

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General description

In this third edition, the chapters have been extensively revised and updated, while 11 new chapters have been added which come from well-established tissue culture laboratories with proven records. The new chapters deal with preparation of substrata, use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. Also included are new and extensive chapters on the isolation, growth, and characterization of neural stem cells and glia progenitor cells.

Table of Contents

In Vitro Assays for Axonal Growth and Targeting, .
Interface Organotypic Hippocampal Slice Cultures,
Slice Cultures for Study of Microglia,
Microexplant Cultures of the Cerebellum,
Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons,
Chick Spinal Somatic Motoneurons in Culture,
Primary Cultures of Sympathetic Ganglia,
Primary Cell Cultures for the Study of Myelination,
Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures,
Culture of Glial Cells from Human Brain Biopsies,
Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium,
Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium,
Olfactory Ensheathing Cell Cultures,
Cultures of Stem Cells of the Central Nervous System,
Aggregating Neural Cell Cultures,
Neural Cell Lines,
Virus Vectors for Gene Therapy of the Nervous System,.
Preparation of Substrata for In Vitro Culture of Neurons,
Serum-Fee Media for Neural Cell Cultures: Adult and Embryonic,
Development of Multiple-Well Plate Biological Assays,
Neural Cell Counting: Cell Number Determination in Microplate Cultures,
Assays for Neuronal Cell Migration,
Colony Culture: Plating Efficiency Assay and Cloning,
Hybridoma Cultures for Production of Antibodies: Purification and Specificity of Antibodies,.
Elimination of Cell Types from Mixed Neural Cell Cultures,
Quantification of Cells in Culture,
Tips for Tissue Culture,
Index.

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