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P8203 Sigma

PIPES

BioXtra, ≥99% (titration)

Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
InChI Key   IHPYMWDTONKSCO-UHFFFAOYSA-N
Quality Level   ELITE
product line   BioXtra
assay   ≥99% (titration)
impurities   Insoluble matter, passes filter test
ign. residue (900°C)   ≤0.5% (as SO4)
loss   ≤0.5% loss on drying, 110°C
useful pH range   6.1 - 7.5
pKa (25 °C)   6.8
mp   >300 °C(lit.)
solubility   1 M NaOH: soluble0.5 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤0.2%
cation traces   Al: ≤0.005%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.005%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.1%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A0.5M/260, 1 M NaOH ≤0.1
  A0.5M/280, 1 M NaOH ≤0.1
Featured Industry   Diagnostic Assay Manufacturing

Description

Packaging

50, 250 g in poly bottle

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

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Buffer Reference Center

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Peer-Reviewed Papers
15

References

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