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P8215 Sigma

Protease Inhibitor Cocktail

for use with fungal and yeast extracts, DMSO solution

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Description

Components

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Application

Optimized and tested for use on fungal and yeast samples. Specifically tested on Saccharomyces cerevisiae cells.

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.

Packaging

1, 5 mL in glass bottle

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Physical form

Solution in DMSO

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
1
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease in...
Keywords: AGE, Adhesion, Aerobic, Affinity chromatography, Alzheimer Disease, Amplification, Angiogenesis, Anti-inflammatory agents, Antibiotics, Antimicrobials, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Autophagy, Biochemistry, Biological processes, Buffers, Cancer, Cardiovascular, Catalysis, Cell biology, Cell disruption, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatin immunoprecipitation, Chromatography, Clinical, Cloning, Cytotoxins, DNA replication, Degradations, Detergents, Diabetes, Diagnostic, Diseases, Dopamine agents, Drug discovery, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gas chromatography, Gastrointestinal, Gene expression, Genetic, Genetics, Gluconeogenesis, Glycosylations, Growth factors, Hormones, Hydration reaction, Hydroxylations, Immobilization, Immunology, Immunoprecipitation, Inflammation, Ligands, Lipid Metabolism, Lipid peroxidation, Mass spectrometry, Medicinal chemistry, Metabolism, Metabolites, Methylations, Microarray Analysis, Microbiology, Microscopy, Molecular biology, Morphogenesis, Neurology, Neuroscience, Neurotransmission, Neurotransmitters, Normal-phase chromatography, Nutrition, Obesity, Oncology, PAGE, Parkinson Disease, Peroxidations, Pharmaceutical, Phosphorylations, Photosynthesis, Physiological Processes, Polymerase chain reaction, Polymorphisms, Proteomics, Purification, Recombination, Reductions, Reproduction, Respiratory, Reversible addition-fragmentation chain transfer polymerizations, Sample preparations, Scanning electron microscopy, Schizophrenia, Separation, Sequencing, Sonication, Transcription, Transduction, Veterinary, Vitamins, Western blot, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Peer-Reviewed Papers
15

References

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