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  • P8234 - Anti-Serine/Threonine Protein Phosphatase 2 A/Bα antibody produced in rabbit

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P8234 Sigma

Anti-Serine/Threonine Protein Phosphatase 2 A/Bα antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   bovine, mouse, human, rat
application(s)   immunocytochemistry: 5-10 μg/mL
  western blot: 5-10 μg/mL using total rat brain homogenate
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPP2R2A(5520)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide (FSQVKGAVDDDVAE) corresponding to the N-terminus (amino acids 14-27) of the protein phosphatase 2A/Bα subunit.

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein phosphatase 2A (PP2A) holozyme consists of a catalytic subunit (C), a structural subunit (A) and a regulatory subunit (B). The subunit B dictates the substrate specificity and is coded by at least 13 genes resulting in 3 distinct classes of subunits - α, β and γ. The B α subunit is targeted to the microtubules, renders substrate specificty and dephosphorylates cyclin-dependent kinase sites. The PP2A holoenzyme has been implicated in various functions such as cell cycle progression, oncogenic transformation and is subjected to tight regulation at the translational level, by post translational modifications and by protein-protein interations.
Anti-Serine/Threonine Protein Phosphatase 2A/Bα recognizes protein phosphatase 2A/Bα?isoforms of 55 kDa.

Physical form

Provided as a 0.2 μm filtered solution in phosphate buffered saline containing 0.08% sodium azide.

Application

Anti-Serine/Threonine Protein Phosphatase 2A/B α may be used for detection at working concentration of 5-10 μg/mL in total rat brain homogenate. The antibody is also suitable for immunocytochemistry at a working concentration of 5-10 μg/mL.

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