|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,|
|mp||258 °C (dec.)(lit.)|
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
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Certificate of Analysis
|Symbol||GHS07, GHS08, GHS09|
|Precautionary statements||P273-P280-P305 + P351 + P338-P501|
|Hazard Codes (Europe)||T,N|
|Risk Statements (Europe)||20/21-25-36-40-43-50/53-68|
|Safety Statements (Europe)||26-36/37-45-61|
|RIDADR||UN 1673 6.1/PG 3|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
Wide cross-reactivity between Anopheles gambiae and Anopheles funestus SG6 salivary proteins supports exploitation of gSG6 as a marker of human exposure to major malaria vectors in tropical Africa. Rizzo C Malaria Journal 10, 206, (2011)
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. Xu H Matrix Biol. 30(1), 16-26, (2011)
Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Boviard, J.H., et al. Clin. Chem. 28, 2423, (1982)
RegBook 1 (1), 1429:B
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