o-Phenylenediamine dihydrochloride
tablet, 10 mg substrate per tablet
DOWNLOAD MSDS (PDF)-
CAS Number 615-28-1
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Linear Formula C6H4(NH2)2·2HCl
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Molecular Weight 181.06
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Beilstein Registry Number 3912045
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MDL number MFCD00012966
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PubChem Substance ID 24898741
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection, |
| Quality Level | PREMIUM |
| form | tablet |
| mp | 258 °C (dec.)(lit.) |
| storage temp. | 2-8°C |
Description
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
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