|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,|
|mp||258 °C (dec.)(lit.)|
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
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Certificate of Analysis
|Symbol||GHS07, GHS08, GHS09|
|Precautionary statements||P273-P280-P305 + P351 + P338-P501|
|Hazard Codes (Europe)||T,N|
|Risk Statements (Europe)||20/21-25-36-40-43-50/53-68|
|Safety Statements (Europe)||26-36/37-45-61|
|RIDADR||UN 1673 6.1 / PGIII|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
The C-terminal segment of the cysteine-rich interdomain of Plasmodium falciparum erythrocyte membrane protein 1 determines CD36 binding and elicits antibodies that inhibit adhesion of parasite-infected erythrocytes. Mo M Infect. Immun. 76(5), 1837-47, (2008)
Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle. Nicholas Juleff et al J. Virol. 83, 3626-36, (2009)
Synthesis, spectroscopic investigations, antimicrobial and DNA binding studies of a new charge transfer complex of o-phenylenediamine with 3,5-dinitrosalicylic acid. Khan IM, Ahmad A, and Ullah MF Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 102, 82-7, (2013)
Spectrofluorimetric determination of trace nitrite with o-phenylenediamine enhanced by hydroxypropyl-β-cyclodextrin. Guo YX, Zhang QF, Shangguang X, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 101, 107-11, (2013)
Syntheses, characterization, biological activities and photophysical properties of lanthanides complexes with a tetradentate Schiff base ligand. Taha ZA, Ajlouni AM, Al Momani W, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 81(1), 570-7, (2011)
Iron(III) complexes of bis (benzimidazol-2-yl) methyl) thiophene-2,5-dicarboxamide: synthesis, spectral and oxidation of o-phenylenediamine. Tyagi N and Mathur P Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 96, 759-67, (2012)
Revisit to the reaction of o-phenylene diamine with thiosemicarbazide to give benzimidazole-2-thione rather than benzotriazine-2-thione and its glycosylation. El Ashry el SH, Aly AA, Aouad MR, et al. Nucleosides Nucleotides Nucleic Acids 29(9), 698-706, (2010)
Synthesis, spectral and thermal studies of the newly hydrogen bonded charge transfer complex of o-phenylenediamine with pi acceptor picric acid. Khan IM and Ahmad A Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 77(2), 437-41, (2010)
Template synthesis, spectroscopic studies, antimicrobial, nematicidal and pesticidal activities of chromium(III) macrocyclic complexes. Masih I, Fahmi N, and Rajkumar J. Enzyme Inhib. Med. Chem. 28(1), 33-40, (2013)
Wide cross-reactivity between Anopheles gambiae and Anopheles funestus SG6 salivary proteins supports exploitation of gSG6 as a marker of human exposure to major malaria vectors in tropical Africa. Rizzo C Malaria Journal 10, 206, (2011)
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. Xu H Matrix Biol. 30(1), 16-26, (2011)
Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents. Boviard, J.H., et al. Clin. Chem. 28, 2423, (1982)
RegBook 1 (1), 1429:B
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