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  • P8333 - Anti-Protein Kinase Cδ antibody produced in rabbit

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P8333 Sigma

Anti-Protein Kinase Cδ antibody produced in rabbit

whole antiserum

Synonym: Anti-PKC δ

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   rat
application(s)   dot blot: 1:50,000
  microarray: suitable
  western blot: 1:10,000 using rat brain extract
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prkcd(170538)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to the C-terminal variable (V5) region (amino acids 662-673) of rat PKC δ coupled to KLH with glutaraldehyde.

General description

PKC is a phospholipid-dependent enzyme that belongs to serine/ threonine (Ser/Thr)-specific protein kinases family. It has a pivotal role in cell growth and differentiation, modulation of neurotransmission, signal transduction and oncogenesis. PKC δ is an isoenzyme is expressed mainly in the brain, heart, lung, liver, spleen, thymus, pancreas, ovary, skin, adrenal gland and rat embryonic fibroblasts. Anti-protein kinase c δ antibody can be used for studying the differential tissue expression and intracellular localization of PKC δ. It can also be used in western blotting and microarray. Rabbit anti-protein kinase c δ antibody reacts specifically with PKC δ peptide conjugated to BSA with 1-ethyl-3-(3-dimethylamino-propyl)- carbodiimide (EDCI). The product is also specific for PKC δ polypeptide (80kD) from rat brain extract.

Application

Anti- protein kinase c δ antibody can be used to identify PKC δisozyme by using different immunochemical assays like- immunoprecipitation, immunohistochemistry, immunoblotting and ELISA. It can also be used in chemiluminescence detection systems to detect PKC δ. Further, the product can also be used in dot-blot immunoassay.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Peer-Reviewed Papers
15

References

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