P8340 Sigma

Protease Inhibitor Cocktail

for use with mammalian cell and tissue extracts, DMSO solution

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Description

Frequently Asked Questions

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Components

AEBSF, 104 mM
Aprotinin, 80 μM
Bestatin, 4 mM
E-64, 1.4 mM
Leupeptin, 2 mM
Pepstatin A, 1.5mM

Application

Use at 1:100 for undiluted blood samples, liver tissue extracts, and CHO cell lysates.

Other Notes

A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatinA, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators.

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of bovine liver.

Specificity

Inhibits, serine, cysteine, aspartic proteases, and amidopeptidases.

Physical form

Solution in DMSO

Price and Availability

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