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  • P8359 - Anti-Serine/Threonine Protein Phosphatase 2 A/B′ pan2 antibody produced in rabbit

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P8359 Sigma

Anti-Serine/Threonine Protein Phosphatase 2 A/B′ pan2 antibody produced in rabbit

~1 mg/mL, IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   rat
application(s)   microarray: suitable
  western blot: 1:500-1:1,000 using total rat brain homogenate
clone   polyclonal
concentration   ~1 mg/mL
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 56 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Ppp2r2b(60660)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide (VSSPHFQVAERALY) corresponding to the N-terminus of the protein phosphatase 2A/B′ pan2 subunit.

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein phosphatase 2A (PP2A) holozyme consists of a catalytic subunit (C), a structural subunit (A) and a regulatory subunit (B). The subunit B dictates the substrate specificity and is coded by at least 13 genes resulting in 3 distinct classes of subunits – B, B’ and B”. Members of the B’/B56/PR61 family regulatory subunits of PP2A determine the subcellular localization, substrate specificity, and catalytic activity of PP2A in a wide range of biological processes. B’/B56/PR61 are reportedly involved in major signaling pathways such as Wnt, Hedgehog and planar cell polarity pathway
Anti-serine/threonine protein phosphatase 2A/B’ pan 2 recognizes an epitope common to the ~56 kDa protein phosphatase 2A B’ subunits from rat brain.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Application

Anti-protein phosphatase 2A/B’ Pan 2 antibody may be used for immunoblotting at a working dilution of 1:500-1:1000 in total rat brain homogenate. The antibody is suitable for protein microarray.

Price and Availability


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