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  • P8458 - Anti-Protein Kinase Cε antibody produced in rabbit

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P8458 Sigma

Anti-Protein Kinase Cε antibody produced in rabbit

whole antiserum

Synonym: Anti-PKC ε

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
species reactivity   rat
application(s)   dot blot: 1:50,000
  microarray: suitable
  western blot: 1:15,000 using rat brain extract
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 90 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Prkce(29340)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide, corresponding to the C-terminal variable (V5) region (amino acids 726-737) of PKC ε.

General description

Protein Kinase C (PKC, 76-93 kD) is a family of ten Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The PKC isoforms are activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC in vivo is thought to be mediated by calpains I and II. There are three subgroups into which the PKC isoforms are divided, classical (conventional), novel and atypical. PKCepsilon and PKCδ are members of the novel subgroup. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. PKCepsilon and PKCδ have sometimes opposite effects. PKCepsilon is most important in stimulation of cell growth and differentiation and modulation of cytoskeleton These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C epsilon?specifically reacts with PKC epsilon (90 kDa) from rat brain extract. It does not react with C-terminal epitopes of other PKC isoforms.

Application

Anti-Protein Kinase C epsilon antibody may be used for detection by immunoblotting at a working dilution of 1:15,000 using rat brain extract. The antibody may also be used for detection of PKCepsilon in whole cell or membrane extracts of HUVEC cells when stimulated with PMA. It may be used in indirectdot blot at a working dilution of 1:50,000. It is also suitable for protein microarray.

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